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Anti-PCCB Antibody (6F980)

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Catalog No. TMAB-10042

Anti-PCCB Antibody (6F980) is a Mouse antibody targeting PCCB. Anti-PCCB Antibody (6F980) can be used in IF, IHC-Fr, IHC-P, WB.

Anti-PCCB Antibody (6F980)

Anti-PCCB Antibody (6F980)

Copy Product Info
😃Good
Catalog No. TMAB-10042
Anti-PCCB Antibody (6F980) is a Mouse antibody targeting PCCB. Anti-PCCB Antibody (6F980) can be used in IF, IHC-Fr, IHC-P, WB.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1507-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4727-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-PCCB Antibody (6F980) is a Mouse antibody targeting PCCB. Anti-PCCB Antibody (6F980) can be used in IF, IHC-Fr, IHC-P, WB.
Ig Type
IgG2a, Kappa
Clone
6F980
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCCB Monoclonal Antibody, Unconjugated (TMAB-10042) at 1: 100 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCCB Monoclonal Antibody, Unconjugated (TMAB-10042) at 1: 100 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCCB Monoclonal Antibody, Unconjugated (TMAB-10042) at 1: 100 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCCB Monoclonal Antibody, Unconjugated (TMAB-10042) at 1: 100 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCCB Monoclonal Antibody, Unconjugated (TMAB-10042) at 1: 100 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCCB Monoclonal Antibody, Unconjugated (TMAB-10042) at 1: 100 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCCB Monoclonal Antibody, Unconjugated (TMAB-10042) at 1: 100 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
8. 25 ug total protein per lane of various lysates (see on figure) probed with PCCB monoclonal antibody, unconjugated (TMAB-10042) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationMitochondrion matrix.
ConstructionRecombinant Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]
Antigen Details
Gene Name
PCCB
Gene ID
Protein Name
Propionyl-CoA carboxylase beta chain, mitochondrial
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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