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Anti-PCCB Antibody (6F980) is a Mouse antibody targeting PCCB. Anti-PCCB Antibody (6F980) can be used in IF, IHC-Fr, IHC-P, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $472 | 7-10 days | 7-10 days |
| Description | Anti-PCCB Antibody (6F980) is a Mouse antibody targeting PCCB. Anti-PCCB Antibody (6F980) can be used in IF, IHC-Fr, IHC-P, WB. |
| Ig Type | IgG2a, Kappa |
| Clone | 6F980 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCCB Monoclonal Antibody, Unconjugated (TMAB-10042) at 1: 100 overnight at 4°C, followed by conjugation to the HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCCB Monoclonal Antibody, Unconjugated (TMAB-10042) at 1: 100 overnight at 4°C, followed by conjugation to the HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCCB Monoclonal Antibody, Unconjugated (TMAB-10042) at 1: 100 overnight at 4°C, followed by conjugation to the HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCCB Monoclonal Antibody, Unconjugated (TMAB-10042) at 1: 100 overnight at 4°C, followed by conjugation to the HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCCB Monoclonal Antibody, Unconjugated (TMAB-10042) at 1: 100 overnight at 4°C, followed by conjugation to the HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCCB Monoclonal Antibody, Unconjugated (TMAB-10042) at 1: 100 overnight at 4°C, followed by conjugation to the HRP and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCCB Monoclonal Antibody, Unconjugated (TMAB-10042) at 1: 100 overnight at 4°C, followed by conjugation to the HRP and DAB staining. 8. 25 ug total protein per lane of various lysates (see on figure) probed with PCCB monoclonal antibody, unconjugated (TMAB-10042) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Mitochondrion matrix. |
| Construction | Recombinant Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010] |
| Gene Name | PCCB |
| Gene ID | |
| Protein Name | Propionyl-CoA carboxylase beta chain, mitochondrial |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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