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Anti-PCBD1 Polyclonal Antibody
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Catalog No. TMAB-10034
Anti-PCBD1 Polyclonal Antibody is a Rabbit antibody targeting PCBD1. Anti-PCBD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-PCBD1 Polyclonal Antibody
Anti-PCBD1 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-10034
Anti-PCBD1 Polyclonal Antibody is a Rabbit antibody targeting PCBD1. Anti-PCBD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-PCBD1 Polyclonal Antibody is a Rabbit antibody targeting PCBD1. Anti-PCBD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat,Cow,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PCBD1) Polyclonal Antibody, Unconjugated (TMAB-10034) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Testis (Mouse) Lysate at 40 μg Primary: Anti-PCBD1 (TMAB-10034) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12 kD Observed band size: 12 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Cytoplasmic and/or nuclear. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes a member of the pterin-4-alpha-carbinolamine dehydratase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein functions as both a dehydratase involved in tetrahydrobiopterin biosynthesis, and as a cofactor for HNF1A-dependent transcription. A deficiency of this enzyme leads to hyperphenylalaninemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Immunogen | KLH conjugated synthetic peptide: human PCBD1 |
| Antigen Species | Human |
| Gene Name | PCBD1 |
| Gene ID | |
| Protein Name | Pterin-4-alpha-carbinolamine dehydratase |
| Uniprot ID | |
| Function | Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity. |
| Molecular Weight | Theoretical: 12 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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