Shopping Cart
Remove All
Your shopping cart is currently empty
Anti-Paxillin Antibody (7F984)
Copy Product Info
😃Good
Catalog No. TMAB-10024
Anti-Paxillin Antibody (7F984) is a Rabbit antibody targeting Paxillin. Anti-Paxillin Antibody (7F984) can be used in WB,ICC/IF,FCM,IHC-P,IHC-Fr,IF.
Anti-Paxillin Antibody (7F984)
Anti-Paxillin Antibody (7F984)
Copy Product Info😃Good
Catalog No. TMAB-10024
Anti-Paxillin Antibody (7F984) is a Rabbit antibody targeting Paxillin. Anti-Paxillin Antibody (7F984) can be used in WB,ICC/IF,FCM,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
Add to Cart
Add to Quotation
In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Resource Download
Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-Paxillin Antibody (7F984) is a Rabbit antibody targeting Paxillin. Anti-Paxillin Antibody (7F984) can be used in WB,ICC/IF,FCM,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 7F984 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. 4% Paraformaldehyde-fixed PC-3 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Paxillin) monoclonal Antibody, unconjugated (TMAB-10024) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control. 2. The PC-3 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Paxillin antibody (TMAB-10024, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Paxillin Monoclonal Antibody, Unconjugated (TMAB-10024) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Paxillin Monoclonal Antibody, Unconjugated (TMAB-10024) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. 25 μg total protein per lane of various lysates (see on figure) probed with Paxillin monoclonal antibody, unconjugated (TMAB-10024) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex. Note=Colocalizes with integrins at the cell periphery. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk). |
| Gene Name | PXN |
| Gene ID | |
| Protein Name | Paxillin |
| Function | Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
Preview
Related Tags: buy Anti-Paxillin Antibody (7F984) | purchase Anti-Paxillin Antibody (7F984) | Anti-Paxillin Antibody (7F984) cost | order Anti-Paxillin Antibody (7F984)
Generate Quote
Catalog No.: Cas No.:
Add
| Size | Quantity | Unit Price | Amount | Operation |
|---|
Generate Quote
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2026 TargetMol Chemicals Inc. All Rights Reserved.