Anti-PAX9 Polyclonal Antibody

Anti-PAX9 Polyclonal Antibody is a Rabbit antibody targeting PAX9. Anti-PAX9 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Tissue/cell: mouse colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-PAX9 Polyclonal Antibody, Unconjugated (TMAB-10021) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-PAX9 Polyclonal Antibody, Unconjugated (TMAB-10021) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Sample:
MCF-7 (Human) Cell Lysate at 30 μg
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-PAX9 (TMAB-10021) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PAX9

Human

PAX9

Paired box protein Pax-9

Transcription factor required for normal development of thymus, parathyroid glands, ultimobranchial bodies, teeth, skeletal elements of skull and larynx as well as distal limbs (By similarity).