Anti-PAX3 Polyclonal Antibody is a Rabbit antibody targeting PAX3. Anti-PAX3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PAX3) Polyclonal Antibody, Unconjugated (TMAB-10012) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-PAX3 Polyclonal Antibody, Unconjugated (TMAB-10012) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Tissue/cell: Human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-PAX3 Polyclonal Antibody, Unconjugated (TMAB-10012) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
4. Sample: Brain (Mouse) Lysate at 40 μg
Primary: Anti-PAX3 (TMAB-10012) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
5. Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Mouse Muscle tissue lysates
Lane 3: Rat Cerebellum tissue lysates
Lane 4: Rat Muscle tissue lysates
Primary: Anti-PAX3 (TMAB-10012) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PAX3

Human

PAX3

Paired box protein Pax-3

Transcription factor that may regulate cell proliferation, migration and apoptosis. Involved in neural development and myogenesis.