Anti-PASD5 Polyclonal Antibody is a Rabbit antibody targeting PASD5. Anti-PASD5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Rabbit)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-PASD5 Polyclonal Antibody, Unconjugated (TMAB-10002) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: Brain (Mouse) Lysate at 40 μg
Primary: Anti-PASD5 (TMAB-10002) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PASD5/NPAS1

Human

NPAS1

Neuronal PAS domain-containing protein 1

PASD5 is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. Studies of a related mouse gene suggest that it functions in neurons. The exact function of PASD5 is unclear, but it may play protective or modulatory roles during late embryogenesis and postnatal development.