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Anti-PARP1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01334
Anti-PARP1 Polyclonal Antibody is a Rabbit antibody targeting PARP1. Anti-PARP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-PARP1 Polyclonal Antibody
Anti-PARP1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01334
Anti-PARP1 Polyclonal Antibody is a Rabbit antibody targeting PARP1. Anti-PARP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-PARP1 Polyclonal Antibody is a Rabbit antibody targeting PARP1. Anti-PARP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Pig,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PARP1) Polyclonal Antibody, Unconjugated (TMAB-01334) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Blank control: 293T. Primary Antibody (green line): Rabbit Anti-PARP1 antibody (TMAB-01334) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 3. Sample: Lane 1: K562 (Human) Cell Lysate at 30 μg Lane 2: SH-SY5Y (Human) Cell Lysate at 30 μg Lane 3: U251 (Human) Cell Lysate at 30 μg Lane 4: Hela (Human) Cell Lysate at 30 μg Primary: Anti-PARP1 (TMAB-01334) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kDa Observed band size: 115 kDa 4. Sample: Testis (Mouse) Lysate at 40 μg Primary: Anti-PARP1 (TMAB-01334) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 112 kDa Observed band size: 112 kDa |
| Application | WB,IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.Nucleus. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human PARP1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | ADP-ribosylation,Base Excision Repair,DNA Damage Recognition |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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