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Anti-PARP1 Antibody (5R212)

😃Good
Catalog No. TMAC-00894
Alias PPOL, Poly-PARP, PARS, PARP-1, PARP, pADPRT-1, ARTD1, ADPRT1, ADPRT 1, ADPRT

Anti-PARP1 Antibody (5R212) is a Rabbit antibody targeting PARP1. Anti-PARP1 Antibody (5R212) can be used in FCM,ICC,IP,WB.

Anti-PARP1 Antibody (5R212)

Anti-PARP1 Antibody (5R212)

😃Good
Catalog No. TMAC-00894Alias PPOL, Poly-PARP, PARS, PARP-1, PARP, pADPRT-1, ARTD1, ADPRT1, ADPRT 1, ADPRT
Anti-PARP1 Antibody (5R212) is a Rabbit antibody targeting PARP1. Anti-PARP1 Antibody (5R212) can be used in FCM,ICC,IP,WB.
Pack SizePriceAvailabilityQuantity
50 μL$2967-10 days
100 μL$4987-10 days
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Instruction Manual of Detection Antibody

Product Introduction

Bioactivity
Description
Anti-PARP1 Antibody (5R212) is a Rabbit antibody targeting PARP1. Anti-PARP1 Antibody (5R212) can be used in FCM,ICC,IP,WB.
SynonymsPPOL, Poly-PARP, PARS, PARP-1, PARP, pADPRT-1, ARTD1, ADPRT1, ADPRT 1, ADPRT
Ig Type
IgG
Clone
5R212
Reactivity
Human
Verified Activity
1. Western blot analysis of Cleaved PARP on diferent lysates using anti-Cleaved PARP antibody at 1/1,000 dilution. Positive control: Lane1: Jurkat, Lane2: A549.
2. Flow cytometric analysis of Hela cells with Cleaved PARP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
verifiedActivityverifiedActivity
Application
FCM
ICC
IP
WB
Recommended Dose
WB: 1:1000-2000; ICC: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundPoly(ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a nuclear DNA-binding zinc finger protein that influences DNA repair, DNA replication, modulation of chromatin structure, and apoptosis. In response to genotoxic stress, PARP-1 catalyzes the transfer of ADP-ribose units from NAD(+) to a number of acceptor molecules including chromatin. PARP-1 recognizes DNA strand interruptions and can complex with RNA and negatively regulate transcription. Actinomycin D- and etoposide-dependent induction of caspases mediates cleavage of PARP-1 into a p89 fragment that traverses into the cytoplasm. Apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus is PARP-1-dependent and is necessary for PARP-1-dependent cell death. PARP-1 deficiencies lead to chromosomal instability due to higher frequencies of chromosome fusions and aneuploidy, suggesting that poly(ADP-ribosyl)ation contributes to the efficient maintenance of genome integrity. This antibody recognizes the apoptosis-specific 89 kDa catalytic domain fragment, but it does not recognize the full-length PARP-1 or the 24 kDa DNA binding domain fragment.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
P09874
Chemical Properties
Molecular WeightTheoretical: 89 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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