Anti-PARP Antibody (1W26) (Synonyms: sPARP-1, PPOL, poly (ADP-ribose) polymerase 1, parp-1, PARP, C80510, ...)

Name: Anti-PARP Antibody (1W26)
Brand: TargetMol
SKU: TMAY-01509
Rating: 4.5 (1 reviews)

Catalog No. TMAY-01509 Copy Product Info

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Anti-PARP Antibody (1W26) is a Mouse antibody targeting PARP. Anti-PARP Antibody (1W26) can be used in IHC-P,IP,WB.

Anti-PARP Antibody (1W26)

Copy Product Info

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Catalog No. TMAY-01509

Synonyms sPARP-1, PPOL, poly (ADP-ribose) polymerase 1, parp-1, PARP, C80510, ARTD1, AI893648, Adprt1, Adprp, 5830444G22Rik

Anti-PARP Antibody (1W26) is a Mouse antibody targeting PARP. Anti-PARP Antibody (1W26) can be used in IHC-P,IP,WB.

Anti-PARP Antibody
(1W26)

Pack Size	Price	USA Stock	Global Stock	Quantity
100 μL	$198	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Product Introduction

Bioactivity

Description	Anti-PARP Antibody (1W26) is a Mouse antibody targeting PARP. Anti-PARP Antibody (1W26) can be used in IHC-P,IP,WB.
Synonyms	sPARP-1, PPOL, poly (ADP-ribose) polymerase 1, parp-1, PARP, C80510, ARTD1, AI893648, Adprt1, Adprp, 5830444G22Rik
Ig Type	Mouse IgG1
Clone	1W26
Reactivity	Human
Specificity	Human PARP-1
Verified Activity	1. Immunochemical staining of human PARP1 in human placenta with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus. 2. Immunochemical staining of human PARP1 in human lymph node with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus. 3. Immunochemical staining of human PARP1 in human breast with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus. 4. Immunochemical staining of human PARP1 in human brain with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus. 5. PARP1 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate -0.5 µL anti-PARP1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PARP1 mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 113 kDa. -Observed band size: 113 kDa. 6. Anti-PARP1 mouse monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:113 kDa. -Observed band size:113 kDa
Application	IHC-P IP WB
Recommended Dose	WB: 1:500-1:1000; IHC-P: 1:100-1:500; IP: 0.2-1 μL/mg of lysate
Antibody Type	Monoclonal
Host Species	Mouse
Construction	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PARP-1 / PARP (rh PARP-1 / PARP; TMPY-01188; NP_001609.2; Met 1-Trp 1014). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification	Protein A
Appearance	Liquid
Formulation	0.2 μm filtered solution in PBS
Research Background	Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 2-3 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrated to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors are thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Related Conjugates and Formulations

Conjucates

Unconjugated

Antigen Details

Immunogen	Recombinant Protein: Human PARP-1 / PARP protein (TMPY-01188)
Antigen Species	Human
Biology Area	Cancer Drug Targets

Chemical Properties

Stability & Storage

Stability & Storage	Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
Transport	Shipping with blue ice.