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Anti-PARP Antibody (1W26)
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Catalog No. TMAY-01509
Alias sPARP-1, PPOL, poly (ADP-ribose) polymerase 1, parp-1, PARP, C80510, ARTD1, AI893648, Adprt1, Adprp, 5830444G22Rik
Anti-PARP Antibody (1W26) is a Mouse antibody targeting PARP. Anti-PARP Antibody (1W26) can be used in WB,IHC-P,IP.
Anti-PARP Antibody (1W26)
Anti-PARP Antibody (1W26)
😃Good
Catalog No. TMAY-01509Alias sPARP-1, PPOL, poly (ADP-ribose) polymerase 1, parp-1, PARP, C80510, ARTD1, AI893648, Adprt1, Adprp, 5830444G22Rik
Anti-PARP Antibody (1W26) is a Mouse antibody targeting PARP. Anti-PARP Antibody (1W26) can be used in WB,IHC-P,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Chemical Properties
| Description | Anti-PARP Antibody (1W26) is a Mouse antibody targeting PARP. Anti-PARP Antibody (1W26) can be used in WB,IHC-P,IP. |
| Alias | sPARP-1, PPOL, poly (ADP-ribose) polymerase 1, parp-1, PARP, C80510, ARTD1, AI893648, Adprt1, Adprp, 5830444G22Rik |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 1W26 |
| Reactivity | Human |
| Verified Activity | 1. Immunochemical staining of human PARP1 in human placenta with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus. 2. Immunochemical staining of human PARP1 in human lymph node with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus. 3. Immunochemical staining of human PARP1 in human breast with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus. 4. Immunochemical staining of human PARP1 in human brain with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus. 5. PARP1 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate -0.5 µL anti-PARP1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PARP1 mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 113 kDa. -Observed band size: 113 kDa. 6. Anti-PARP1 mouse monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:113 kDa. -Observed band size:113 kDa |
| Application | WB,IHC-P,IP |
| Recommended Dose | WB: 1:500-1:1000; IHC-P: 1:100-1:500; IP: 0.2-1 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PARP-1 / PARP (rh PARP-1 / PARP; TMPY-01188; NP_001609.2; Met 1-Trp 1014). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 2-3 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrated to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors are thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human PARP-1 / PARP protein (TMPY-01188) |
| Antigen Species | Human |
| Biology Area | Cancer Drug Targets |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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