Anti-PAR6 Antibody (2R536) is a Rabbit antibody targeting PAR6. Anti-PAR6 Antibody (2R536) can be used in WB,IP,FCM.

IgG

2R536

Human,Mouse,Rat

1. Western blot analysis of Jurkat cell lysate. Using PAR6 (TMAB-09984) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-PAR6 antibody (TMAB-09984, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:1000-2000; IP: 1:20-50; FCM: 1:50-100

Monoclonal

A synthesized peptide: human PAR6 alpha

Human

PARD6A

Partitioning defective 6 homolog alpha

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.