Anti-PAN3 Polyclonal Antibody is a Rabbit antibody targeting PAN3. Anti-PAN3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Mouse (predicted:Human,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PAN3) Polyclonal Antibody, Unconjugated (TMAB-09958) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PAN3

Human

PAN3

PAN2-PAN3 deadenylation complex subunit PAN3

Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by poly(A)-binding protein (PABP), which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3-5 exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5-3 exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with PABP and to miRNA targets via its interaction with GW182 family proteins.