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Anti-PAI1 Antibody (8G921)

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Catalog No. TMAB-09935

Anti-PAI1 Antibody (8G921) is a Rabbit antibody targeting PAI1. Anti-PAI1 Antibody (8G921) can be used in FCM, WB.

Anti-PAI1 Antibody (8G921)

Anti-PAI1 Antibody (8G921)

Copy Product Info
😃Good
Catalog No. TMAB-09935
Anti-PAI1 Antibody (8G921) is a Rabbit antibody targeting PAI1. Anti-PAI1 Antibody (8G921) can be used in FCM, WB.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1507-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4737-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-PAI1 Antibody (8G921) is a Rabbit antibody targeting PAI1. Anti-PAI1 Antibody (8G921) can be used in FCM, WB.
Ig Type
IgG
Clone
8G921
Reactivity
Human
Verified Activity
1. 25 ug total protein per lane of various lysates (see on figure) probed with PAI1 monoclonal antibody, unconjugated (TMAB-09935) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The HUVEC (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-PAI1 antibody (TMAB-09935, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
Application
Recommended Dose
FCM=1:50-100; WB=1:500-2000
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted
Tissue SpecificityFound in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation50mM Tris-Glycine (pH7.4), 0.15M NaCl, 40% Glycerol, 0.02% Proclin300 and 0.05% BSA.
Concentration1mg/ml
Research BackgroundPAI1 (plasminogen activator inhibitor 1) is originally cloned from human endothelial cell (Pannekoek 1986, Ginsburg 1986) and rat hepatoma cell 3 cDNA libraries. As a member of the serpin family of serine protease inhibitors, PAI1 inhibits both tissue type plasminogen activator (tPA) and urokinase type plasminogen activator (uPA). High PAI1 levels are associated with an increased risk of thromboembolic disease while PAI1 deficiency may represent an inherited autosomal recessive bleeding disorder.
Antigen Details
Immunogen
A synthesized peptide: human PAI 1
Antigen Species
Human
Gene Name
SERPINE1
Gene ID
Protein Name
Plasminogen activator inhibitor 1
Uniprot ID
Function
Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.
Chemical Properties
Molecular WeightTheoretical: 43 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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