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Anti-PAI-1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01327
Anti-PAI-1 Polyclonal Antibody is a Rabbit antibody targeting PAI-1. Anti-PAI-1 Polyclonal Antibody can be used in WB,ICC/IF.
Anti-PAI-1 Polyclonal Antibody
Anti-PAI-1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01327
Anti-PAI-1 Polyclonal Antibody is a Rabbit antibody targeting PAI-1. Anti-PAI-1 Polyclonal Antibody can be used in WB,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,ICC/IF Reactivity: Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit,Sheep) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit,Sheep) |
| Verified Activity | 1. A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PAI1) polyclonal Antibody, Unconjugated (TMAB-01327) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PAI1) polyclonal Antibody, Unconjugated (TMAB-01327) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 3. Sample: Lane 1: Mouse Lung tissue lysates Lane 2: Rat Lung tissue lysates Primary: Anti-PAI1 (TMAB-01327) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 46 kDa |
| Application | WB,ICC/IF |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:100 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | PAI1 (plasminogen activator inhibitor 1) is originally cloned from human endothelial cell (Pannekoek 1986, Ginsburg 1986) and rat hepatoma cell 3 cDNA libraries. As a member of the serpin family of serine protease inhibitors, PAI1 inhibits both tissue type plasminogen activator (tPA) and urokinase type plasminogen activator (uPA). High PAI1 levels are associated with an increased risk of thromboembolic disease while PAI1 deficiency may represent an inherited autosomal recessive bleeding disorder. |
| Immunogen | KLH conjugated synthetic peptide: human PAI-1 |
| Antigen Species | Human |
| Gene ID | 5054 |
| Uniprot ID |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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