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Anti-PACRG Polyclonal Antibody

Catalog No. TMAB-09916 Copy Product Info
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Anti-PACRG Polyclonal Antibody is a Rabbit antibody targeting PACRG. Anti-PACRG Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-PACRG Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-09916

Anti-PACRG Polyclonal Antibody is a Rabbit antibody targeting PACRG. Anti-PACRG Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-PACRG Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2217-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-PACRG Polyclonal Antibody is a Rabbit antibody targeting PACRG. Anti-PACRG Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Rat (predicted:Human,Mouse,Dog,Horse,Rabbit,Sheep,Zebrafish)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PACRG) Polyclonal Antibody, Unconjugated (TMAB-09916) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PACRG) Polyclonal Antibody, Unconjugated (TMAB-09916) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Tissue SpecificityExpressed in all immune tissues, spleen, lymph nodes, thymus, tonsils, leukocyte and bone marrow. Expressed also in heart, brain, skeletal muscle, kidney, lung and pancreas. Expressed in primary Schwann cells and very weakly by monocyte-derived macrophages the primary host cells of Mycobacterium leprae, the causative agent of leprosy. Component of Lewy bodies, intraneuronal inclusions found in the brain of Parkinson disease patients.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe deduced 257 amino acid protein PACRG (Parkin co-regulated gene) shows potential links to the ubiquitin/proteasome system. PACRG and Parkin are attached in a head-to-head arrangement on opposite DNA strands and share a common 5' flanking promoter region. The PACRG gene maps to chromosome 6q26; Northern blot analysis detects PACRG expression in all tissues examined except placenta. Using a positional cloning strategy in 197 Vietnamese leprosy simplex families (i.e. families with two unaffected parents and one affected child), significant connections between leprosy and 17 markers in the 5' regulatory region that PARK2 and PACRG share were observed. Possession of two or more of the 17 risk alleles is highly predictive of leprosy. PACRG is a gene located very close to parkin, in reverse orientation on the chromosome. It is thought to be co-transcribed with parkin by a bi-directional promoter between the two genes.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PACRG
Antigen Species
Human
Gene Name
PACRG
Gene ID
Protein Name
Parkin coregulated gene protein
Uniprot ID
Function
PACRG is a gene located very close to parkin, in reverse orientation on the chromosome. It is thought to be co-transcribed with parkin by a bi-directional promoter between the two genes.
Chemical Properties
Molecular WeightTheoretical: 33 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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