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Anti-P73 Polyclonal Antibody
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Catalog No. TMAB-09902
Anti-P73 Polyclonal Antibody is a Rabbit antibody targeting P73. Anti-P73 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-P73 Polyclonal Antibody
Anti-P73 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-09902
Anti-P73 Polyclonal Antibody is a Rabbit antibody targeting P73. Anti-P73 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-P73 Polyclonal Antibody is a Rabbit antibody targeting P73. Anti-P73 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Cow,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human esophagus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p73 alpha) Polyclonal Antibody, Unconjugated (TMAB-09902) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p73 alpha) Polyclonal Antibody, Unconjugated (TMAB-09902) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Sample: Lane 1: Human HeLa cell lysates Lane 2: Human MOLT4 cell lysates Primary: Anti-P73 (TMAB-09902) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kDa Observed band size: 73 kDa 4. Sample: Lane 1: Mouse Spleen tissue lysates Lane 2: Mouse Kidney tissue lysates Primary: Anti-P73 (TMAB-09902) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kDa Observed band size: 73 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm. Note=Accumulates in the nucleus in response to DNA damage. |
| Tissue Specificity | Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It maps to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Many transcript variants resulting from alternative splicing and/or use of alternate promoters have been found for this gene, but the biological validity and the full-length nature of some variants have not been determined. [provided by RefSeq, Feb 2011]. |
| Immunogen | KLH conjugated synthetic peptide: mouse p73 alpha |
| Antigen Species | Mouse |
| Gene Name | TP73 |
| Gene ID | |
| Protein Name | Tumor protein p73 |
| Uniprot ID | |
| Function | Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. [COFACTOR] Binds 1 zinc ion per subunit |
| Molecular Weight | Theoretical: 70 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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