Anti-P2X2 Polyclonal Antibody is a Rabbit antibody targeting P2X2. Anti-P2X2 Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Rabbit,GuineaPig)

1. Sample: DU145 (Human) Cell Lysate at 30 μg
Primary: Anti-P2X2 (TMAB-09857) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:52 kD
Observed band size: 67 kD
2. Blank control: MCF7.
Primary Antibody (green line): Rabbit Anti-P2X2 antibody (TMAB-09857)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 2 μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human ATP receptor

Human

P2RX2

P2X purinoceptor 2

Binding of this ligand-gated ion channel to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle.