Anti-P-cadherin Polyclonal Antibody is a Rabbit antibody targeting P-cadherin. Anti-P-cadherin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Cow,Rabbit,GuineaPig)

1. Overlay histogram showing Mouse SP2/0 stained with TMAB-10032-PE (red line). The cells were fixed with 1% paraformaldehyde (10 min). The cells were then incubated with the antibody (TMAB-10032-PE, 2ug/1x106cells) for 30 min at 22-25°C. Isotype control antibody (black line) was rabbit IgG (2ug/1x106cells) used under the same conditions. Acquisition of >5,000 events was performed.
2. Tissue/cell: U251 cells; 4% Paraformaldehyde-fixed;
Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-P-cadherin Polyclonal Antibody, Unconjugated (TMAB-10032) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml, blue) was used to stain the cell nuclei
3. Image provided by Independent Validation (badge 029741). Histogram of human DU145 and human LNCaP cells stained with Rabbit Anti-P-cadherin Polyclonal Antibody (orange) (TMAB-10032 at 1:100), isotype control antibody (green), secondary antibody only (blue) and unstained (red)
4. Sample: MCF-7 (Human) Cell Lysate at 30 μg
Primary: Anti-P-cadherin (TMAB-10032) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 90 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human P-cadherin

Human

CDH3

Cadherin-3

Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.