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Anti-ox-LDL Polyclonal Antibody

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Catalog No. TMAB-01308
Alias ox-LDL: LDL(Copper oxidized), oxidized low density lipoprotein, MDA oxidized low density lipoprotein, MDA oxidized LDL, Low density lipoprotein, Cu2SO4 oxidized low density lipoprotein

Anti-ox-LDL Polyclonal Antibody is a Rabbit antibody targeting ox-LDL. Anti-ox-LDL Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-ox-LDL Polyclonal Antibody

Anti-ox-LDL Polyclonal Antibody

😃Good
Catalog No. TMAB-01308Alias ox-LDL: LDL(Copper oxidized), oxidized low density lipoprotein, MDA oxidized low density lipoprotein, MDA oxidized LDL, Low density lipoprotein, Cu2SO4 oxidized low density lipoprotein
Anti-ox-LDL Polyclonal Antibody is a Rabbit antibody targeting ox-LDL. Anti-ox-LDL Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-ox-LDL Polyclonal Antibody is a Rabbit antibody targeting ox-LDL. Anti-ox-LDL Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Aliasox-LDL: LDL(Copper oxidized), oxidized low density lipoprotein, MDA oxidized low density lipoprotein, MDA oxidized LDL, Low density lipoprotein, Cu2SO4 oxidized low density lipoprotein
Ig Type
IgG
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ox-LDL) Polyclonal Antibody, Unconjugated (TMAB-01308) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Blank control: A431. Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (TMAB-01308) Dilution: 3 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3 μg/test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (TMAB-01308)
Dilution: 2 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
IHC-P,IHC-Fr,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundLow-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. Low-density lipoprotein oxidised with Cu2SO4.
Antigen Details
Immunogen
Full length protein from human plasma
Antigen Species
Human
Gene ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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