Anti-OTX1/2 Antibody (6K799) is a Rabbit antibody targeting OTX1/2. Anti-OTX1/2 Antibody (6K799) can be used in WB,IHC-P,IHC-Fr,IP,ChIP,IF.

IgG

6K799

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with OTX1/2 monoclonal antibody, unconjugated (TMAB-09835) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OTX1/2 Monoclonal Antibody, Unconjugated (TMAB-09835) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OTX1/2 Monoclonal Antibody, Unconjugated (TMAB-09835) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OTX1/2 Monoclonal Antibody, Unconjugated (TMAB-09835) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OTX1/2 Monoclonal Antibody, Unconjugated (TMAB-09835) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OTX1/2 Monoclonal Antibody, Unconjugated (TMAB-09835) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OTX1/2 Monoclonal Antibody, Unconjugated (TMAB-09835) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (red, Goat Anti-Rabbit IgG H&L-BF594), DAPI (blue) was used to stain the cell nuclei.
8. Paraformaldehyde-fixed, paraffin embedded human testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OTX1/2 Monoclonal Antibody, Unconjugated (TMAB-09835) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (red, Goat Anti-Rabbit IgG H&L-BF594), DAPI (blue) was used to stain the cell nuclei.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IP: 1:20-50; ChIP: 1:20-50; IF: 1:50-200

Monoclonal

A synthesized peptide: human OTX1

Human

OTX1

Homeobox protein OTX1

Function: Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.