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Anti-Osteoprotegerin Polyclonal Antibody 2
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Catalog No. TMAB-01301
Anti-Osteoprotegerin Polyclonal Antibody 2 is a Rabbit antibody targeting Osteoprotegerin. Anti-Osteoprotegerin Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Osteoprotegerin Polyclonal Antibody 2
Anti-Osteoprotegerin Polyclonal Antibody 2
😃Good
Catalog No. TMAB-01301
Anti-Osteoprotegerin Polyclonal Antibody 2 is a Rabbit antibody targeting Osteoprotegerin. Anti-Osteoprotegerin Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat (predicted:Dog,Rabbit) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (OPG) Polyclonal Antibody, Unconjugated (TMAB-01301) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (OPG) Polyclonal Antibody, Unconjugated (TMAB-01301) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Lane 1: Raji (Human) Cell Lysate at 30 μg Lane 2: U2os (Human) Cell Lysate at 30 μg Lane 3: Heart (Mouse) Lysate at 40 μg Lane 4: Heart (Rat) Lysate at 40 μg Lane 5: Liver (Mouse) Lysate at 40 μg Lane 6: Liver (Rat) Lysate at 40 μg Lane 7: Kidney (Mouse) Lysate at 40 μg Lane 8: Kidney (Rat) Lysate at 40 μg Primary: Anti-OPG (TMAB-01301) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Osteoprotegerin (OPG, or osteoclastogenesis inhibitory factor) is a secretory glycoprotein belonging to TNF receptor superfamily. Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. OPG acts as a soluble factor in the regulation of bone mass and may be beneficial in the treatment of osteoporosis with increased osteoclast activity. OPG consists of 401 amino acids with a molecular weight of 44 kDa as a monomer and 90 kDa as a disulphide-linked dimer. |
| Immunogen | KLH conjugated synthetic peptide: human OPG |
| Antigen Species | Human |
| Gene ID | 4982 |
| Uniprot ID | |
| Biology Area | Inflammatory mediators,Blood,Bone,TNF Superfamily,Obesity,Bone,TNF |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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