Anti-Osteoprotegerin Polyclonal Antibody is a Rabbit antibody targeting Osteoprotegerin. Anti-Osteoprotegerin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Cow)

1. Tissue/cell: rat tibia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Osteoprotegerin Polyclonal Antibody, Unconjugated (TMAB-01300) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Heart (Mouse) Lysate at 40 μg
Kidney (Mouse) Lysate at 40 μg
Liver (Mouse) Lysate at 40 μg
Primary: Anti-OPg (TMAB-01300) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 50 kDa
3. Sample:
Lane 1: Raji (Human) Cell Lysate at 30 μg
Lane 2: U251 (Human) Cell Lysate at 30 μg
Primary: Anti-OPG (TMAB-01300) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human OPG

Human

Inflammatory mediators,Blood,Bone,TNF Superfamily,Obesity,Bone,TNF