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Anti-Osteoprotegerin Polyclonal Antibody is a Rabbit antibody targeting Osteoprotegerin. Anti-Osteoprotegerin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-Osteoprotegerin Polyclonal Antibody is a Rabbit antibody targeting Osteoprotegerin. Anti-Osteoprotegerin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | tumor necrosis factor receptor superfamily member 11b, TR1, PDB5, OPG, OCIF, MGC29565 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Dog,Cow) |
Verified Activity | 1. Tissue/cell: rat tibia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Osteoprotegerin Polyclonal Antibody, Unconjugated (TMAB-01300) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Heart (Mouse) Lysate at 40 μg Kidney (Mouse) Lysate at 40 μg Liver (Mouse) Lysate at 40 μg Primary: Anti-OPg (TMAB-01300) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 50 kDa 3. Sample: Lane 1: Raji (Human) Cell Lysate at 30 μg Lane 2: U251 (Human) Cell Lysate at 30 μg Primary: Anti-OPG (TMAB-01300) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Secreted. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Osteoprotegerin (OPG, or osteoclastogenesis inhibitory factor) is a secretory glycoprotein belonging to TNF receptor superfamily. Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. OPG acts as a soluble factor in the regulation of bone mass and may be beneficial in the treatment of osteoporosis with increased osteoclast activity. OPG consists of 401 amino acids with a molecular weight of 44 kDa as a monomer and 90 kDa as a disulphide-linked dimer. |
Immunogen | KLH conjugated synthetic peptide: human OPG |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Inflammatory mediators,Blood,Bone,TNF Superfamily,Obesity,Bone,TNF |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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