Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-Osteopontin Polyclonal Antibody

😃Good
Catalog No. TMAB-01297

Anti-Osteopontin Polyclonal Antibody is a Rabbit antibody targeting Osteopontin. Anti-Osteopontin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-Osteopontin Polyclonal Antibody

Anti-Osteopontin Polyclonal Antibody

😃Good
Catalog No. TMAB-01297
Anti-Osteopontin Polyclonal Antibody is a Rabbit antibody targeting Osteopontin. Anti-Osteopontin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$373 7-10 days
200 μL$529 7-10 days
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-Osteopontin Polyclonal Antibody is a Rabbit antibody targeting Osteopontin. Anti-Osteopontin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Rat
Verified Activity
1. Sample: A549 Cell Lysate at 40 μg
Primary: Anti-ostepontin (TMAB-01297) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 50 kDa
2. Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 μg
Lane 2: A549 (Human) Cell Lysate at 30 μg
Lane 3: U251 (Human) Cell Lysate at 30 μg
Primary: Anti-Osteopontin at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60-65 kDa
Observed band size: 60 kDa
3. Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Osteopontin Polyclonal Antibody, Unconjugated (TMAB-01297) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-Osteopontin antibody (TMAB-01297)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:500-2000; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundOsteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human OPN
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: buy Anti-Osteopontin Polyclonal Antibody | purchase Anti-Osteopontin Polyclonal Antibody | Anti-Osteopontin Polyclonal Antibody cost | order Anti-Osteopontin Polyclonal Antibody