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Anti-Osteopontin Antibody (7E998)
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Catalog No. TMAB-09824
Anti-Osteopontin Antibody (7E998) is a Rabbit antibody targeting Osteopontin. Anti-Osteopontin Antibody (7E998) can be used in IHC-P,WB,IHC-Fr,IF.
Anti-Osteopontin Antibody (7E998)
Anti-Osteopontin Antibody (7E998)
Copy Product Info😃Good
Catalog No. TMAB-09824
Anti-Osteopontin Antibody (7E998) is a Rabbit antibody targeting Osteopontin. Anti-Osteopontin Antibody (7E998) can be used in IHC-P,WB,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 200 μg | $1,060 | 7-10 days | 7-10 days | |
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days | |
| 200 μL | $754 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Osteopontin Antibody (7E998) is a Rabbit antibody targeting Osteopontin. Anti-Osteopontin Antibody (7E998) can be used in IHC-P,WB,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 7E998 |
| Reactivity | Human |
| Verified Activity | 1. Sample: Lane 1: Recombinant human Osteopontin protein, C-His (HEK293) Primary: Anti-Osteopontin (TMAB-09824) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 63 kDa 2. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Osteopontin Monoclonal Antibody, Unconjugated (TMAB-09824) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Osteopontin Monoclonal Antibody, Unconjugated (TMAB-09824) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Osteopontin Monoclonal Antibody, Unconjugated (TMAB-09824) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Osteopontin Monoclonal Antibody, Unconjugated (TMAB-09824) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Osteopontin Monoclonal Antibody, Unconjugated (TMAB-09824) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Osteopontin Monoclonal Antibody, Unconjugated (TMAB-09824) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; WB: 1:500-2000; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Tissue Specificity | Bone. Found in plasma. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma. |
| Immunogen | Recombinant Protein: human Osteopontin protein |
| Antigen Species | Human |
| Gene Name | SPP1 |
| Gene ID | |
| Protein Name | Osteopontin |
| Uniprot ID | |
| Function | Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity). |
| Molecular Weight | Theoretical: 35 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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