Anti-Osteocalcin Polyclonal Antibody is a Rabbit antibody targeting Osteocalcin. Anti-Osteocalcin Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.

IgG

Mouse (predicted:Human,Rat,Dog,Cow,Horse)

1. Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse osteosarcoma labeled with Anti-Osteocalcin Polyclonal Antibody, Unconjugated (TMAB-01295) at 1:100 followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: bone of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Osteocalcin Polyclonal Antibody, Unconjugated (TMAB-01295) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human BGP

Human

BGLAP

Osteocalcin

Bone,Osteogenesis

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.