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Anti-Osteocalcin Polyclonal Antibody
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Catalog No. TMAB-01295
Alias Osteocalcin, Gamma-carboxyglutamic acid-containing protein, Bone Gla protein (BGP), BGLAP
Anti-Osteocalcin Polyclonal Antibody is a Rabbit antibody targeting Osteocalcin. Anti-Osteocalcin Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.
Anti-Osteocalcin Polyclonal Antibody
Anti-Osteocalcin Polyclonal Antibody
😃Good
Catalog No. TMAB-01295Alias Osteocalcin, Gamma-carboxyglutamic acid-containing protein, Bone Gla protein (BGP), BGLAP
Anti-Osteocalcin Polyclonal Antibody is a Rabbit antibody targeting Osteocalcin. Anti-Osteocalcin Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Concentration:1.0 mg/mL
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Osteocalcin Polyclonal Antibody is a Rabbit antibody targeting Osteocalcin. Anti-Osteocalcin Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P. |
| Synonyms | Osteocalcin, Gamma-carboxyglutamic acid-containing protein, Bone Gla protein (BGP), BGLAP |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Cow,Horse) |
| Verified Activity | 1. Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse osteosarcoma labeled with Anti-Osteocalcin Polyclonal Antibody, Unconjugated (TMAB-01295) at 1:100 followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: bone of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Osteocalcin Polyclonal Antibody, Unconjugated (TMAB-01295) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.   |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite. |
| Immunogen | KLH conjugated synthetic peptide: human BGP |
| Antigen Species | Human |
| Gene Name | BGLAP |
| Gene ID | |
| Protein Name | Osteocalcin |
| Uniprot ID | |
| Biology Area | Bone,Osteogenesis |
| Function | Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. |
| Molecular Weight | Theoretical: 11 kDa. |
| Color | Transparent |
| Appearance | Liquid |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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