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Anti-Osteocalcin Antibody (3V479)
Catalog No. TMAB-09818 Copy Product Info
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Anti-Osteocalcin Antibody (3V479) is a Mouse antibody targeting Osteocalcin. Anti-Osteocalcin Antibody (3V479) can be used in WB,IHC-Fr,IF,IHC-P.
Anti-Osteocalcin Antibody (3V479)
Copy Product Info🥰Excellent
Catalog No. TMAB-09818
Anti-Osteocalcin Antibody (3V479) is a Mouse antibody targeting Osteocalcin. Anti-Osteocalcin Antibody (3V479) can be used in WB,IHC-Fr,IF,IHC-P.
Anti-Osteocalcin Antibody
(3V479)
(3V479)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-Osteocalcin Antibody (3V479) is a Mouse antibody targeting Osteocalcin. Anti-Osteocalcin Antibody (3V479) can be used in WB,IHC-Fr,IF,IHC-P. |
| Ig Type | IgG |
| Clone | 3V479 |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Osteocalcin) Monoclonal Antibody, Unconjugated (TMAB-09818) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 2. Sample: Lane 1: Recombinant human Osteocalcin, C-hFc (HEK293) Primary: Anti-Osteocalcin (TMAB-09818) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 11 kDa Observed band size: 35.5 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-Fr: 1:400-800; IF: 1:100-500; IHC-P: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Secreted. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite. |
| Immunogen | Recombinant Protein: human Osteocalcin protein |
| Antigen Species | Human |
| Gene Name | BGLAP |
| Gene ID | |
| Protein Name | Osteocalcin |
| Uniprot ID | |
| Function | Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. |
| Molecular Weight | Theoretical: 11 kDa. Actual: 35.5 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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