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Anti-Osteoactivin/GPNMB Polyclonal Antibody
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Catalog No. TMAB-01294
Anti-Osteoactivin/GPNMB Polyclonal Antibody is a Rabbit antibody targeting Osteoactivin/GPNMB. Anti-Osteoactivin/GPNMB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Osteoactivin/GPNMB Polyclonal Antibody
Anti-Osteoactivin/GPNMB Polyclonal Antibody
😃Good
Catalog No. TMAB-01294
Anti-Osteoactivin/GPNMB Polyclonal Antibody is a Rabbit antibody targeting Osteoactivin/GPNMB. Anti-Osteoactivin/GPNMB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Osteoactivin/GPNMB Polyclonal Antibody is a Rabbit antibody targeting Osteoactivin/GPNMB. Anti-Osteoactivin/GPNMB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Pig,Cow,Horse,GuineaPig) |
| Verified Activity | 1. Tissue/cell: rat lung tissue (melanoma); 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-GPNMB/Osteoactivin Polyclonal Antibody, Unconjugated (TMAB-01294) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Lane 1: Recombinant human GPNMB protein, C-His (HEK293) Primary: Anti-GPNMB (TMAB-01294) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kDa Observed band size: 65 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human GPNMB |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Tumor-associated antigens,Macrophage / Inflamm. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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