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Anti-ORP150 Antibody (6Q346)
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Catalog No. TMAC-02925
Alias ORP-150, ORP150, hypoxia up-regulated 1, HSP12A, GRP-170, Grp170
Anti-ORP150 Antibody (6Q346) is a Rabbit antibody targeting ORP150. Anti-ORP150 Antibody (6Q346) can be used in WB,IHC,FCM.
Anti-ORP150 Antibody (6Q346)
Anti-ORP150 Antibody (6Q346)
😃Good
Catalog No. TMAC-02925Alias ORP-150, ORP150, hypoxia up-regulated 1, HSP12A, GRP-170, Grp170
Anti-ORP150 Antibody (6Q346) is a Rabbit antibody targeting ORP150. Anti-ORP150 Antibody (6Q346) can be used in WB,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-ORP150 Antibody (6Q346) is a Rabbit antibody targeting ORP150. Anti-ORP150 Antibody (6Q346) can be used in WB,IHC,FCM. |
| Synonyms | ORP-150, ORP150, hypoxia up-regulated 1, HSP12A, GRP-170, Grp170 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of ORP150 on mouse prostate tissue lysate using anti-ORP150 antibody at 1/5,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ORP150 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-ORP150 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin. 6. Flow cytometric analysis of HepG2 cells with ORP150 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:5000-10000; IHC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | ORP150, also known as HYOU1 (hypoxia upregulated 1), is a 999 amino acid protein that localizes to the lumen of the endoplasmic reticulum (ER) and is a member of the heat shock protein 70 (HSP 70) family. ORP150 is highly expressed in tissues that have well-developed ERs and a large number of secretory proteins (such as liver and pancreas). It is expressed at lower levels in kidney and brain, and plays an essential role in cytoprotective cellular responses to hypoxia (oxygen deprivation). Specifically, ORP150 participates in protein folding and secretion in the ER and functions to protect cells from hypoxia-induced apoptosis, thereby playing a crucial role in cell survival. ORP150 expression is upregulated in a variety of tumors, such as breast cancer, suggesting an important role in tumorigenesis. The gene encoding ORP150 has two translation initiation sites, resulting in a truncated transcript that lacks an ER signal peptide, but is thought to function as a housekeeping protein in the cytoplasm. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: human ORP150 aa 800-950 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 111 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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