Anti-ORP1 Antibody (5R818) is a Rabbit antibody targeting ORP1. Anti-ORP1 Antibody (5R818) can be used in WB,ICC/IF,FCM.

IgG

5R818

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with ORP1 monoclonal antibody, unconjugated (TMAB-09800) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The A549 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ORP1 antibody (TMAB-09800, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100

Monoclonal

A synthesized peptide: human ORP1

Human

OSBPL1A

Oxysterol-binding protein-related protein 1

Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate.