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Anti-ORP1 Antibody (5R818)

Catalog No. TMAB-09800 Copy Product Info
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Anti-ORP1 Antibody (5R818) is a Rabbit antibody targeting ORP1. Anti-ORP1 Antibody (5R818) can be used in WB,ICC/IF,FCM.

Anti-ORP1 Antibody (5R818)

Copy Product Info
🥰Excellent
Catalog No. TMAB-09800

Anti-ORP1 Antibody (5R818) is a Rabbit antibody targeting ORP1. Anti-ORP1 Antibody (5R818) can be used in WB,ICC/IF,FCM.

Anti-ORP1 Antibody
(5R818)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1507-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4737-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-ORP1 Antibody (5R818) is a Rabbit antibody targeting ORP1. Anti-ORP1 Antibody (5R818) can be used in WB,ICC/IF,FCM.
Ig Type
IgG
Clone
5R818
Reactivity
Human,Mouse,Rat
Verified Activity
1. 25 ug total protein per lane of various lysates (see on figure) probed with ORP1 monoclonal antibody, unconjugated (TMAB-09800) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The A549 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ORP1 antibody (TMAB-09800, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
Application
Recommended Dose
WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationLate endosome. Note=Colocalizes with RAB7A, RAB9A and LAMP1 in late endosomes.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundBinds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate.Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A.
Antigen Details
Immunogen
A synthesized peptide: human ORP1
Antigen Species
Human
Gene Name
OSBPL1A
Gene ID
Protein Name
Oxysterol-binding protein-related protein 1
Uniprot ID
Function
Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate.
Chemical Properties
Molecular WeightTheoretical: 108 kDa. Actual: 50, 108 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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