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Anti-Optimedin Polyclonal Antibody
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Catalog No. TMAB-09784
Anti-Optimedin Polyclonal Antibody is a Rabbit antibody targeting Optimedin. Anti-Optimedin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Optimedin Polyclonal Antibody
Anti-Optimedin Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-09784
Anti-Optimedin Polyclonal Antibody is a Rabbit antibody targeting Optimedin. Anti-Optimedin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-Optimedin Polyclonal Antibody is a Rabbit antibody targeting Optimedin. Anti-Optimedin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Optimedin) Polyclonal Antibody, Unconjugated (TMAB-09784) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Eye (Mouse) Lysate at 40 μg Cerebellum (Mouse) Lysate at 40 μg Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-Optimedin (TMAB-09784) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Tissue Specificity | In the eye, expressed in trabecular meshwork and neural retina; in non-ocular tissues, expressed in brain and lung. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | OLFM3 is a 478 amino acid protein that interacts with myocilin. Myocilin is an extracellular protein that plays a key role in the actomyosin system and is responsible for controlling intraocular pressure. OLFM3 is a secreted protein that contains an olfactomedin-like (OLF) domain, an approximately 260 amino acid motif commonly found in secreted glycoproteins. OLFM3 localizes to the Golgi apparatus of the cell and is highly expressed in both eye and brain tissue. Mutations in the gene that encodes OLFM3 may cause severe glaucoma, a condition in which increased intraocular pressure within the eyeball causes loss of eye sight. |
| Immunogen | KLH conjugated synthetic peptide: human OLFM3/Optimedin |
| Antigen Species | Human |
| Gene Name | OLFM3 |
| Gene ID | |
| Protein Name | Noelin-3 |
| Uniprot ID |
| Molecular Weight | Theoretical: 52 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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