Anti-OMA1 Polyclonal Antibody is a Rabbit antibody targeting OMA1. Anti-OMA1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Pig,Cow,Horse,Rabbit,Zebrafish,Cat)

1. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (OMA1) Polyclonal Antibody, Unconjugated (TMAB-09772) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Sample: TT Cell (Human) Lysate at 40 μg
Primary: Anti-OMA1 (TMAB-09772) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 55 kD
3. Sample: Heart (Mouse) Lysate at 40 μg
Primary: Anti-OMA1 (TMAB-09772) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 55 kD
4. Sample: Kidney (Mouse) Lysate at 40 μg
Primary: Anti-OMA1 (TMAB-09772) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 55 kD
5. Sample: Heart (Rat) Lysate at 40 μg
Primary: Anti-OMA1 (TMAB-09772) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 55 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human OMA1

Human

OMA1

Metalloendopeptidase OMA1, mitochondrial

Mitochondrial protease.