Anti-OLIG3 Antibody (8G710) is a Rabbit antibody targeting OLIG3. Anti-OLIG3 Antibody (8G710) can be used in WB,IHC-P,IHC-Fr,IF,ICC/IF.

IgG

8G710

Human

1. Western blot analysis of 293T cell lysate. Using OLIG3 (TMAB-09769) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OLIG3 Monoclonal Antibody, Unconjugated (TMAB-09769) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OLIG3 Monoclonal Antibody, Unconjugated (TMAB-09769) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. 4% Paraformaldehyde-fixed U-87MG (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (OLIG3) monoclonal Antibody, unconjugated (TMAB-09769) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ICC/IF: 1:50-200

Monoclonal

A synthesized peptide: human Oligo3

Human

OLIG3

Oligodendrocyte transcription factor 3

May determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.