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Anti-OIT3 Polyclonal Antibody (Synonyms: Oncoprotein induced transcript 3, OIT3, LZP, Liver specific ZP domai...)

Catalog No. TMAB-01283 Copy Product Info
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Anti-OIT3 Polyclonal Antibody is a Rabbit antibody targeting OIT3. Anti-OIT3 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

Anti-OIT3 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-01283
Synonyms Oncoprotein induced transcript 3, OIT3, LZP, Liver specific ZP domain containing protein, Liver specific zona pellucida domain containing protein

Anti-OIT3 Polyclonal Antibody is a Rabbit antibody targeting OIT3. Anti-OIT3 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

Anti-OIT3 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-OIT3 Polyclonal Antibody is a Rabbit antibody targeting OIT3. Anti-OIT3 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
SynonymsOncoprotein induced transcript 3, OIT3, LZP, Liver specific ZP domain containing protein, Liver specific zona pellucida domain containing protein
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Cow)
Verified Activity
1. Sample:
Liver (Mouse) Lysate at 40 μg
Primary: Anti-OIT3 (TMAB-01283) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
2. Paraformaldehyde-fixed, paraffin embedded (human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (OIT3) Polyclonal Antibody, Unconjugated (TMAB-01283) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (OIT3) Polyclonal Antibody, Unconjugated (TMAB-01283) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
verifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus envelope. Can be secreted into blood.
Tissue SpecificityLiver-specific. Expressed only in the hepatocytes. Down-regulated in hepatocellular carcinoma (HCC) and HCC cell lines.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundOIT3 contains one EGF-like domain and one ZP domain. It may be involved in hepatocellular function and development. OIT3 is liver-specific and is expressed only in the hepatocytes; down-regulated in hepatocellular carcinoma (HCC) and HCC cell lines. There are two named isoforms.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human OIT3
Antigen Species
Human
Gene Name
OIT3
Gene ID
Protein Name
Oncoprotein-induced transcript 3 protein
Uniprot ID
Function
May be involved in hepatocellular function and development.
Chemical Properties
Molecular WeightTheoretical: 51 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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