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Anti-OGDH Antibody (9O643)

Catalog No. TMAB-09754 Copy Product Info
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Anti-OGDH Antibody (9O643) is a Mouse antibody targeting OGDH. Anti-OGDH Antibody (9O643) can be used in WB,ICC/IF.

Anti-OGDH Antibody (9O643)

Copy Product Info
🥰Excellent
Catalog No. TMAB-09754

Anti-OGDH Antibody (9O643) is a Mouse antibody targeting OGDH. Anti-OGDH Antibody (9O643) can be used in WB,ICC/IF.

Anti-OGDH Antibody
(9O643)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1497-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4717-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-OGDH Antibody (9O643) is a Mouse antibody targeting OGDH. Anti-OGDH Antibody (9O643) can be used in WB,ICC/IF.
Ig Type
IgG2b/Kappa
Clone
9O643
Reactivity
Human (predicted:Rat)
Verified Activity
1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2 h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: Raji, 2: HepG2, 3: MCF-7, 4: Rat heart
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 116 kDa
Observed MW: 116 kDa
2. Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-09754
Application
Recommended Dose
WB: 1:500-2000; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationMitochondrion matrix.
ConstructionRecombinant Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]
Antigen Details
Gene Name
OGDH
Gene ID
Protein Name
2-oxoglutarate dehydrogenase, mitochondrial
Function
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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