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Anti-Oct4 Antibody (5L999)

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Catalog No. TMAB-09745

Anti-Oct4 Antibody (5L999) is a Rabbit antibody targeting Oct4. Anti-Oct4 Antibody (5L999) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Oct4 Antibody (5L999)

Anti-Oct4 Antibody (5L999)

😃Good
Catalog No. TMAB-09745
Anti-Oct4 Antibody (5L999) is a Rabbit antibody targeting Oct4. Anti-Oct4 Antibody (5L999) can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1487-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4717-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Oct4 Antibody (5L999) is a Rabbit antibody targeting Oct4. Anti-Oct4 Antibody (5L999) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Clone
5L999
Reactivity
Human (predicted:Mouse)
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.Note=Expressed in a diffuse and slightly punctuate pattern.
Tissue SpecificityExpressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Concentration1mg/ml
Research BackgroundExpression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.
Antigen Details
Immunogen
A synthesized peptide: human Oct 4
Antigen Species
Human
Gene Name
POU5F1
Gene ID
Protein Name
POU domain, class 5, transcription factor 1
Uniprot ID
Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Chemical Properties
Molecular WeightTheoretical: 39 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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