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Anti-Oct4 Antibody (4H630)
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Catalog No. TMAY-00055
Alias OTF4, OTF-3, OTF3, Oct-4, Oct4, Oct3/4, Oct-3, Oct3, MGC22487
Anti-Oct4 Antibody (4H630) is a Mouse antibody targeting Oct4. Anti-Oct4 Antibody (4H630) can be used in FCM.
Anti-Oct4 Antibody (4H630)
Anti-Oct4 Antibody (4H630)
😃Good
Catalog No. TMAY-00055Alias OTF4, OTF-3, OTF3, Oct-4, Oct4, Oct3/4, Oct-3, Oct3, MGC22487
Anti-Oct4 Antibody (4H630) is a Mouse antibody targeting Oct4. Anti-Oct4 Antibody (4H630) can be used in FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Oct4 Antibody (4H630) is a Mouse antibody targeting Oct4. Anti-Oct4 Antibody (4H630) can be used in FCM. |
| Alias | OTF4, OTF-3, OTF3, Oct-4, Oct4, Oct3/4, Oct-3, Oct3, MGC22487 |
| Ig Type | Monoclonal Mouse IgG2a |
| Clone | 4H630 |
| Reactivity | Human |
| Verified Activity | Flow cytometric analysis of Oct4 in human embryonic stem (ES) cells. Human embryonic stem (ES) cells were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation/Permeabilization buffer (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-Oct4 monoclonal antibody, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM |
| Recommended Dose | FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The POU5F1 gene, which encodes the POU domain, class 5, transcription factor 1 (also known as Oct-4), is expressed in embryonic stem cells where it regulates pluripotency and proliferation.The POU5F1 gene encodes one of the "core" transcription factors necessary to establish and maintain pluripotency in mammals. Its function depends on its precise level of expression, so its transcription has to be tightly regulated. POU5F1 is a key regulator of self-renewal and differentiation in embryonic stem cells and may be associated with initiation, promotion, and progression in cancer. POU5F1 is a transcription factor and master regulator of cell pluripotency with indispensable roles in early embryo development and cell lineage specification. The role of embryonic POU5F1 in blastocyst formation and cell lineage specification differs between mammalian species. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide corresponding to the C-terminus of the Human Oct4. |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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