Anti-OCT2 Polyclonal Antibody is a Rabbit antibody targeting OCT2. Anti-OCT2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-OCT2 Polyclonal Antibody, Unconjugated (TMAB-09742) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: A549 Cell lysate at 30 μg;
Primary: Anti-OCT2 (TMAB-09742) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgGat 1: 3000 dilution;
Predicted band size: 51 kD
Observed band size: 51 kD
3. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-OCT2 Polyclonal Antibody, Unconjugated (TMAB-09742) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
4. Sample:
Lane 1: Mouse Lymph node tissue lysates
Lane 2: Rat Lymph node tissue lysates
Primary: Anti-OCT2 (TMAB-09742) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: mouse OCT2

Mouse

POU2F2

POU domain, class 2, transcription factor 2

Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.