Anti-Oct-4B/OCT4B-190 Polyclonal Antibody is a Rabbit antibody targeting Oct-4B/OCT4B-190. Anti-Oct-4B/OCT4B-190 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow)

1. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Oct-4B OCT4B-190) Polyclonal Antibody, Unconjugated (TMAB-09746) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Oct-4B OCT4B-190) Polyclonal Antibody, Unconjugated (TMAB-09746) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Oct-4B OCT4B-190) Polyclonal Antibody, Unconjugated (TMAB-09746) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Heart (Mouse) Lysate at 40 μg
Lane 3: Cerebellum (Mouse) Lysate at 40 μg
Lane 4: Testis (Mouse) Lysate at 40 μg
Lane 5: Cerebrum (Rat) Lysate at 40 μg
Lane 6: Heart (Rat) Lysate at 40 μg
Lane 7: Cerebellum (Rat) Lysate at 40 μg
Primary: Anti-Oct-4B/OCT4B-190 (TMAB-09746) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 47 kD
5. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Oct-4B OCT4B-190) Polyclonal Antibody, Unconjugated (TMAB-09746) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human OCT4

Human

POU5F1

POU domain, class 5, transcription factor 1

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.