Anti-OCT-1 Antibody (1I190) is a Mouse antibody targeting OCT-1. Anti-OCT-1 Antibody (1I190) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

1I190

Human

1. Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (OCT-1) Polyclonal Antibody, Unconjugated (TMAB-09736) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Sample: Raji (Human) Cell Lysate at 30 μg
Primary: Anti-OCT-1 (TMAB-09736) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 76 kD
Observed band size: 80 kD
3. Sample: Hela (Human) Cell Lysate at 30 μg
Primary: Anti-OCT-1 (TMAB-09736) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 76 kD
Observed band size: 80 kD

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human Oct1

Human

POU2F1

POU domain, class 2, transcription factor 1

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR By similarity. In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.