Antibody Type: Rabbit Polyclonal

Application: WB,FCM

Reactivity: Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,GuineaPig)

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,GuineaPig)

1. The blue histogram is unstained cells (293FT cells).
The Wathet Blue histogram is cells stained with secondary antibody alone.
The Orange histogram is cells stained with rabbit IgG isotype control antibody
plus secondary antibody.
The green histogram is cells stained with Rabbit Anti-OCLN antibody (TMAB-01281) plus secondary antibody (Concentraion 1: 20).
2. Sample:
Lane 1: A549 (Human) Cell Lysate at 30 μg
Lane 2: MCF-7 (Human) Cell Lysate at 30 μg
Lane 3: A431 (Human) Cell Lysate at 30 μg
Lane 4: HepG2 (Human) Cell Lysate at 30 μg
Lane 5: Hela (Human) Cell Lysate at 30 μg
Primary: Anti-OCLN (TMAB-01281) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 65/38/28 kDa
3. Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-OCLN antibody (TMAB-01281)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,FCM

WB: 1:500-2000; FCM: 2µg/Test

Polyclonal

KLH conjugated synthetic peptide: mouse OCLN

Mouse

18260

Tight Junctions