Anti-OCLN Polyclonal Antibody is a Rabbit antibody targeting OCLN. Anti-OCLN Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,GuineaPig)

1. Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-Occludin antibody (TMAB-01280)
Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Sample:
Lane 1: A549 (Human) Cell Lysate at 30 μg
Lane 2: HepG2 (Human) Cell Lysate at 30 μg
Lane 3: Hela (Human) Cell Lysate at 30 μg
Primary: Anti-Occludin (TMAB-01280) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65/55/38/28 kDa
Observed band size: 62/52/40/25 kDa
3. Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Occludin antibody (TMAB-01280)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. Blank control (blue): 293t (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Occludin/FITC Conjugated antibody (TMAB-01280/FITC), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC (orange),used under the same conditions.

WB,FCM

WB: 1:500-2000; FCM: 2µg/Test

Polyclonal

KLH conjugated synthetic peptide: human Occludin

Human

Tight Junctions