Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse,Rat

IgG

Human,Mouse,Rat

1. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-OAT-1/SLC22A6 (human) Polyclonal Antibody, Unconjugated (TMAB-01278) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Kidney (Human) lysate at 30 μg;
Brain (Rat) lysate at 30 μg;
Primary: Anti-OAT-1/SLC22A6 (human) (TMAB-01278) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000 dilution;
Predicted band size: 62 kDa
Observed band size: 61 kDa
3. Sample: Raji Cell lysate at 30 μg;
Primary: Anti-SLC22A6 (TMAB-01278) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000 dilution;
Predicted band size: 62 kDa
Observed band size: 62 kDa
4. Sample:
Liver (Mouse) Lysate at 40 μg
Primary: Anti-SLC22A6 (TMAB-01278) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human OCT-1

Human

9356

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