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Anti-OAT-1 Polyclonal Antibody

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Catalog No. TMAB-01278
Alias solute carrier family 22(organic anion transporter), member 6, SLC22A6, S22A6, ROAT1, PAHT, Organic anion transporter 1, Orctl1, OAT1, Oat, NKT, mOat1, MGC45260, MGC124962, HOAT1, FLJ55736

Anti-OAT-1 Polyclonal Antibody is a Rabbit antibody targeting OAT-1. Anti-OAT-1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

Anti-OAT-1 Polyclonal Antibody

Anti-OAT-1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01278Alias solute carrier family 22(organic anion transporter), member 6, SLC22A6, S22A6, ROAT1, PAHT, Organic anion transporter 1, Orctl1, OAT1, Oat, NKT, mOat1, MGC45260, MGC124962, HOAT1, FLJ55736
Anti-OAT-1 Polyclonal Antibody is a Rabbit antibody targeting OAT-1. Anti-OAT-1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3727-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-OAT-1 Polyclonal Antibody is a Rabbit antibody targeting OAT-1. Anti-OAT-1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
Synonymssolute carrier family 22(organic anion transporter), member 6, SLC22A6, S22A6, ROAT1, PAHT, Organic anion transporter 1, Orctl1, OAT1, Oat, NKT, mOat1, MGC45260, MGC124962, HOAT1, FLJ55736
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-OAT-1/SLC22A6 (human) Polyclonal Antibody, Unconjugated (TMAB-01278) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Kidney (Human) lysate at 30 μg;
Brain (Rat) lysate at 30 μg;
Primary: Anti-OAT-1/SLC22A6 (human) (TMAB-01278) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000 dilution;
Predicted band size: 62 kDa
Observed band size: 61 kDa
3. Sample: Raji Cell lysate at 30 μg;
Primary: Anti-SLC22A6 (TMAB-01278) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000 dilution;
Predicted band size: 62 kDa
Observed band size: 62 kDa
4. Sample:
Liver (Mouse) Lysate at 40 μg
Primary: Anti-SLC22A6 (TMAB-01278) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi-pass membrane protein.
Tissue SpecificityStrongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundRecent advances in molecular biology have identified three organic anion transporter families: the organic anion transporter (OAT) family encoded by SLC22A, the organic anion transporting peptide (OATP) family encoded by SLC21A (SLCO), and the multidrug resistance-associated protein (MRP) family encoded by ABCC. These families play critical roles in the transepithelial transport of organic anions in the kidneys as well as in other tissues such as the liver and brain. Among these families, the OAT family plays the central role in renal organic anion transport. Knowledge of these three families at the molecular level, such as substrate selectivity, tissue distribution, and gene localization, is rapidly increasing.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human OCT-1
Antigen Species
Human
Gene Name
SLC22A6
Gene ID
Protein Name
Solute carrier family 22 member 6
Uniprot ID
Biology Area
Channels
Function
Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS) (By similarity). Mediates the sodium-independent uptake of p-aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid (By similarity). PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate.
Chemical Properties
Molecular WeightTheoretical: 62 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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