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Anti-NUP88 Polyclonal Antibody

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Catalog No. TMAB-09712

Anti-NUP88 Polyclonal Antibody is a Rabbit antibody targeting NUP88. Anti-NUP88 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-NUP88 Polyclonal Antibody

Anti-NUP88 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-09712
Anti-NUP88 Polyclonal Antibody is a Rabbit antibody targeting NUP88. Anti-NUP88 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2227-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5297-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-NUP88 Polyclonal Antibody is a Rabbit antibody targeting NUP88. Anti-NUP88 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Sample: testis (Mouse) Lysate at 40 μg
Primary: Anti-NUP88 (TMAB-09712) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 85 kD
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NUP88) Polyclonal Antibody, Unconjugated (TMAB-09712) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NUP88) Polyclonal Antibody, Unconjugated (TMAB-09712) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus, nuclear pore complex.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundNUP88 is a nucleoporin that is an essential component of the nuclear pore complex. It interacts with NUP214/CAN, and is phosphorylated following DNA damage, possibly via the ATM/ATR pathway. NUP88 is also a tumour marker as it is overexpressed in a large number of malignant neoplasms and precancerous dysplasias.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NUP88
Antigen Species
Human
Gene Name
NUP88
Gene ID
Protein Name
Nuclear pore complex protein Nup88
Function
Essential component of nuclear pore complex.
Chemical Properties
Molecular WeightTheoretical: 85 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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