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Anti-NUP153 Antibody (4X360)
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Catalog No. TMAC-02906
Alias NUP 153, Nucleoporin Nup153, Nuclear pore complex protein Nup153, 153 kDa nucleoporin
Anti-NUP153 Antibody (4X360) is a Rabbit antibody targeting NUP153. Anti-NUP153 Antibody (4X360) can be used in FCM,ICC/IF,WB.
Anti-NUP153 Antibody (4X360)
Anti-NUP153 Antibody (4X360)
😃Good
Catalog No. TMAC-02906Alias NUP 153, Nucleoporin Nup153, Nuclear pore complex protein Nup153, 153 kDa nucleoporin
Anti-NUP153 Antibody (4X360) is a Rabbit antibody targeting NUP153. Anti-NUP153 Antibody (4X360) can be used in FCM,ICC/IF,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Chemical Properties
| Description | Anti-NUP153 Antibody (4X360) is a Rabbit antibody targeting NUP153. Anti-NUP153 Antibody (4X360) can be used in FCM,ICC/IF,WB. |
| Synonyms | NUP 153, Nucleoporin Nup153, Nuclear pore complex protein Nup153, 153 kDa nucleoporin |
| Ig Type | IgG |
| Clone | 4X360 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of NUP153 on K562 (1) and HepG2 (2) cell lysate using anti-NUP153 antibody at 1/500 dilution. 2. ICC staining NUP153 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining NUP153 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining NUP153 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Nuclear pore complexes (NPCs) are the channels for the bi-directional movement of macromolecules between the nucleus and cytoplasm, and contain more than 100 different subunits. Many of them belong to a family called nucleoporins, which are characterized by the presence of O-linked N-acetylglucosamine moieties and a distinctive pentapeptide repeat (XFXFG). Nup153 is a peripheral NPC component that is implicated in protein and RNP transport and in the interaction of NPCs with the nuclear lamina. Nup153 contains a unique N-terminal region, a central domain consisting of four to five zinc fingers and a C-terminal region containing about 30 irregularly spaced FXFG repeats. Nup153 is cleaved by caspases during apoptosis. Nup153 interacts wtih TAP, which is essential for mRNA export, and associates with chromatin towards the end of anaphase, in parallel with the inner nuclear membrane protein LAP2. Nup153 is involved in NPC assembly, in anchoring NPCs within the nuclear envelope and in mediating specific nuclear import events. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 154 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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