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Anti-NUDCD1 Polyclonal Antibody
Catalog No. TMAB-09684 Copy Product Info
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Anti-NUDCD1 Polyclonal Antibody is a Rabbit antibody targeting NUDCD1. Anti-NUDCD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-NUDCD1 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-09684
Anti-NUDCD1 Polyclonal Antibody is a Rabbit antibody targeting NUDCD1. Anti-NUDCD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-NUDCD1 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-NUDCD1 Polyclonal Antibody is a Rabbit antibody targeting NUDCD1. Anti-NUDCD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat,Dog,Cow,Horse,Rabbit,Zebrafish,Sheep) |
| Verified Activity | 1. Sample: Thymus (Mouse) Lysate at 40 μg Primary: Anti-NUDCD1 (TMAB-09684) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 63 kD 2. Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NUDCD1) Polyclonal Antibody, Unconjugated (TMAB-09684) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The NUDCD1 gene encodes the 583 amino acid CML66 protein which demonstrates expression in a variety of solid tumor cell lines and leukemias. Expression of CML66 in normal tissues is restricted to testis and heart. The CML66 antibody is found in sera of 18-38% of patients with lung cancer, melanoma or prostate cancer. These findings indicate that CML66 may be immunogenic in a wide variety of malignancies and may be a target for antigen-specific immunotherapy. A short isoform of CML66, designated CML66-S, is a product of alternative splicing. CML66-S has the same C-terminus as full-lenghth CML66 (also designated CML66-L), but has a unique N-terminus. CML66-S shows predominant expression in testis, and is also expressed in very low levels in tumor cells, while CML66 is expressed in tumor cells and testis. |
| Immunogen | KLH conjugated synthetic peptide: human NUDCD1 |
| Antigen Species | Human |
| Gene Name | NUDCD1 |
| Gene ID | |
| Protein Name | NudC domain-containing protein 1 |
| Uniprot ID | |
| Function | Isoform 1 is specifically expressed in leukemias and a variety of solid tumor cell lines and is also detected in testis and heart. Isoform 2 is predominantly expressed in testis and weakly expressed in tumor cells. |
| Molecular Weight | Theoretical: 67 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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