Anti-Nucleoporin p62 Polyclonal Antibody is a Rabbit antibody targeting Nucleoporin p62. Anti-Nucleoporin p62 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Blank control (Black line): Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-Nucleoporin p62 antibody (TMAB-09678)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30 min; Antibody incubation with (Nucleoporin p62) Polyclonal/MonoclonalAntibody, Unconjugated (TMAB-09678) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Nucleoporin p62

Human

NUP62

Nuclear pore glycoprotein p62

Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex.