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Anti-Nucleolar protein 3 Polyclonal Antibody is a Rabbit antibody targeting Nucleolar protein 3. Anti-Nucleolar protein 3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
| Description | Anti-Nucleolar protein 3 Polyclonal Antibody is a Rabbit antibody targeting Nucleolar protein 3. Anti-Nucleolar protein 3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat,Dog,Cow,Rabbit) |
| Verified Activity | Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Nucleolar protein 3 Polyclonal Antibody, Unconjugated (TMAB-09670) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm and Nucleus > nucleolus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD)bcontaining molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf 1, caspase 9 and caspase 2. Apoptosis repressor with CARD is a CARD domain containing protein that interacts with caspase 2 and 8 to inhibit enzymatic activity of caspase 8. Apoptosis repressor with CARD suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR 1, and DR3. The mRNA of Apoptosis repressor with CARD is primarily expressed in skeletal muscle and cardiac tissue. The nuclear isoform (1/Nop30) may be involved in RNA splicing and the cytoplasmic isoform (2/Myp) may inhibit apoptosis. |
| Immunogen | KLH conjugated synthetic peptide: mouse Nucleolar protein 3/Apoptosis repressor with CARD |
| Antigen Species | Mouse |
| Gene Name | NOL3 |
| Gene ID | |
| Protein Name | Nucleolar protein 3 |
| Uniprot ID | |
| Function | May be involved in RNA splicing (By similarity). |
| Molecular Weight | Theoretical: 25 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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