Anti-NTR3 Polyclonal Antibody is a Rabbit antibody targeting NTR3. Anti-NTR3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,GuineaPig)

1. Sample: Brain (Rat) Lysate at 40 μg
Primary: Anti-NTR3 (TMAB-09662) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 84 kD
Observed band size: 84 kD
2. Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-NTR3 Polyclonal Antibody, Unconjugated (TMAB-09662) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NTR3) Polyclonal Antibody, Unconjugated (TMAB-09662) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Sample: Heart (Mouse) Lysate at 40 μg
Primary: Anti-NTR3 (TMAB-09662) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84 kD
Observed band size: 84 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Sortilin/NTR3/Gp95

Human

SORT1

Sortilin

Functions as a sorting receptor in the Golgi compartmentand as a clearance receptor on the cell surface. Required forprotein transport from the Golgi apparatus to the lysosomes by apathway that is independent of the mannose-6-phosphate receptor(M6PR). Also required for protein transport from the Golgiapparatus to the endosomes. Promotes neuronal apoptosis bymediating endocytosis of the proapoptotic precursor forms of BDNF(proBDNF) and NGFB (proNGFB). Also acts as a receptor forneurotensin. May promote mineralization of the extracellular matrixduring osteogenic differentiation by scavenging extracellular LPL.Probably required in adipocytes for the formation of specializedstorage vesicles containing the glucose transporter SLC2A4/GLUT4(GLUT4 storage vesicles, or GSVs). These vesicles provide a stablepool of SLC2A4 and confer increased responsiveness to insulin. Mayalso mediate transport from the endoplasmic reticulum to the Golgi.