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Anti-NT5C3A Antibody (5M344)

😃Good
Catalog No. TMAY-02656
Alias UMPH1, UMPH, PSN1, POMP, PN-I, P5N-1, P5'N-1, P5N1, p36, NT5C3, hUMP1, HSPC233, cN-III

Anti-NT5C3A Antibody (5M344) is a Rabbit antibody targeting NT5C3A. Anti-NT5C3A Antibody (5M344) can be used in WB,IP.

Anti-NT5C3A Antibody (5M344)

Anti-NT5C3A Antibody (5M344)

😃Good
Catalog No. TMAY-02656Alias UMPH1, UMPH, PSN1, POMP, PN-I, P5N-1, P5'N-1, P5N1, p36, NT5C3, hUMP1, HSPC233, cN-III
Anti-NT5C3A Antibody (5M344) is a Rabbit antibody targeting NT5C3A. Anti-NT5C3A Antibody (5M344) can be used in WB,IP.
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100 μL$269 7-10 days
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Product Introduction

Bioactivity
Description
Anti-NT5C3A Antibody (5M344) is a Rabbit antibody targeting NT5C3A. Anti-NT5C3A Antibody (5M344) can be used in WB,IP.
AliasUMPH1, UMPH, PSN1, POMP, PN-I, P5N-1, P5'N-1, P5N1, p36, NT5C3, hUMP1, HSPC233, cN-III
Ig Type
Monoclonal Rabbit IgG
Clone
5M344
Reactivity
Human
Verified Activity
1. Anti-NT5C3 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HepG2 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:38 kDa.

-Observed band size:39 kDa.

2. NT5C3 was immunoprecipitated using:

-Lane A:0.5 mg 293T Whole Cell Lysate.

-Lane B:0.5 mg Jurkat Whole Cell Lysate

-0.5 µL anti-NT5C3 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-NT5C3 rabbit monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 38 kDa.

-Observed band size: 38 kDa
Application
WB,IP
Recommended Dose
WB: 1:500-1:1000; IP: 1-2 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human NT5C3A / UMPH1 (rh NT5C3A / UMPH1; NP_001002010; Met12-Leu297).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundNT5C3A (5'-Nucleotidase, Cytosolic IIIA) is a Protein Coding gene. This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. NT5C3A expression required both an intronic IFN-stimulated response element and the IFN-stimulated transcription factor IRF1. Overexpression of NT5C3A, but not of its catalytic mutants, suppressed IL-8 production by HEK293 cells. NT5C3A-stimulated sirtuin activity resulted in deacetylation of histone H3 and the NF-kappaB subunit RelA (also known as p65), both of which were associated with the proximal region of the Il8 promoter, thus repressing the transcription of Il8 Together.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human NT5C3A / UMPH1 Protein
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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