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Anti-NT5C3A Antibody (5M344)
😃Good
Catalog No. TMAY-02656
Alias UMPH1, UMPH, PSN1, POMP, PN-I, P5N-1, P5'N-1, P5N1, p36, NT5C3, hUMP1, HSPC233, cN-III
Anti-NT5C3A Antibody (5M344) is a Rabbit antibody targeting NT5C3A. Anti-NT5C3A Antibody (5M344) can be used in WB,IP.
Anti-NT5C3A Antibody (5M344)
Anti-NT5C3A Antibody (5M344)
😃Good
Catalog No. TMAY-02656Alias UMPH1, UMPH, PSN1, POMP, PN-I, P5N-1, P5'N-1, P5N1, p36, NT5C3, hUMP1, HSPC233, cN-III
Anti-NT5C3A Antibody (5M344) is a Rabbit antibody targeting NT5C3A. Anti-NT5C3A Antibody (5M344) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, IP Reactivity: Human |
| Alias | UMPH1, UMPH, PSN1, POMP, PN-I, P5N-1, P5'N-1, P5N1, p36, NT5C3, hUMP1, HSPC233, cN-III |
| Ig Type | Rabbit IgG |
| Clone | 5M344 |
| Reactivity | Human |
| Verified Activity | 1. Anti-NT5C3 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:38 kDa. -Observed band size:39 kDa. 2. NT5C3 was immunoprecipitated using: -Lane A:0.5 mg 293T Whole Cell Lysate. -Lane B:0.5 mg Jurkat Whole Cell Lysate -0.5 µL anti-NT5C3 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-NT5C3 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 38 kDa. -Observed band size: 38 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:1000; IP: 1-2 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human NT5C3A / UMPH1 (rh NT5C3A / UMPH1; NP_001002010; Met12-Leu297). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | NT5C3A (5'-Nucleotidase, Cytosolic IIIA) is a Protein Coding gene. This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. NT5C3A expression required both an intronic IFN-stimulated response element and the IFN-stimulated transcription factor IRF1. Overexpression of NT5C3A, but not of its catalytic mutants, suppressed IL-8 production by HEK293 cells. NT5C3A-stimulated sirtuin activity resulted in deacetylation of histone H3 and the NF-kappaB subunit RelA (also known as p65), both of which were associated with the proximal region of the Il8 promoter, thus repressing the transcription of Il8 Together. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human NT5C3A / UMPH1 Protein |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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