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Anti-NSE/ENO2 Antibody (8L64) is a Rabbit antibody targeting NSE/ENO2. Anti-NSE/ENO2 Antibody (8L64) can be used in ICC,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $297 | 7-10 days | 7-10 days | |
| 100 μL | $497 | 7-10 days | 7-10 days |
| Description | Anti-NSE/ENO2 Antibody (8L64) is a Rabbit antibody targeting NSE/ENO2. Anti-NSE/ENO2 Antibody (8L64) can be used in ICC,IHC,WB. |
| Synonyms | NSE, HEL-S-279, enolase 2 (γ, neuronal), enolase 2 (gamma, neuronal) |
| Ig Type | IgG |
| Clone | 8L64 |
| Reactivity | Human,Mouse,Rat,zebrafish |
| Verified Activity | 1. Western blot analysis of NSE on different lysates using anti-NSE antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2, Lane 2: Hela, Lane 3: 293. 2. Western blot analysis of NSE on hybrid fish (crucian-carp) brain tissue lysates using anti-NSE antibody at 1/500 dilution. 3. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NSE antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NSE antibody. Counter stained with hematoxylin. 5. ICC staining NSE in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining NSE in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ![]() ![]() ![]() ![]() ![]() ![]() |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified, α Enolase, β Enolase and γ Enolase. α Enolase expression has been detected on most tissues, whereas β Enolase is expressed predominantly in muscle tissue and γ Enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 47 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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