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Anti-NSE/ENO2 Antibody (3X783) is a Mouse antibody targeting NSE/ENO2. Anti-NSE/ENO2 Antibody (3X783) can be used in IHC-P,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μL | $199 | 7-10 days | 7-10 days |
| Description | Anti-NSE/ENO2 Antibody (3X783) is a Mouse antibody targeting NSE/ENO2. Anti-NSE/ENO2 Antibody (3X783) can be used in IHC-P,WB. |
| Synonyms | NSE, HEL-S-279, enolase 2 (γ, neuronal), enolase 2 (gamma, neuronal) |
| Ig Type | Mouse IgG1 |
| Clone | 3X783 |
| Reactivity | Human |
| Specificity | Human Enolase 2/ENO2 |
| Verified Activity | 1. Immunochemical staining of human ENO2 in human pancreas with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. 2. Immunochemical staining of human ENO2 in human brain with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. 3. Anti-ENO2 mouse monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: SH-SY5Y Whole Cell Lysate. -Lane C: Hela Whole Cell Lysate. -Lane D: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:47 kDa. -Observed band size:50 kDa |
| Application | |
| Recommended Dose | WB: 1:500-1:2000; IHC-P: 1:100-1:500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ENO2 (rh ENO2; TMPY-02646; P09104; Met1-Leu434). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The combination of silencing ENO2 and 2-deoxyglucose (2-DG) synergistically inhibited leukemia cell survival. ENO2 may be a biological marker for monitoring chemotherapeutic efficacy and relapse in ALL. Reduced ENO2 expression may be a biomarker for a subset of autistic children. Neuron specific enolase (ENO2, gamma-enolase) has been used as a biomarker to help identify neuroendocrine differentiation in breast cancer. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human ENO2 Protein (TMPY-02646) |
| Antigen Species | Human |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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